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mouse anti phb2  (Proteintech)


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    Proteintech mouse anti phb2
    Mouse Anti Phb2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+phb2/Prohibitin+2+Antibody/10__3390_slash_ijms262210852-119-15-17
    Average 94 stars, based on 60 article reviews
    mouse anti phb2 - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: SLP2/PHB Aggregates in ALS Mouse Models and Patients: Implications Beyond CHCHD10-Associated Motor Neuron Disease
    Article Snippet: .. Floating sections were incubated overnight at 4 ◦C with primary antibodies diluted in blocking buffer: mouse anti-PHB2 (Proteintech, Rosemont, IL, USA, 66424-1-Ig; 1/400), rabbit anti-SLP2 (Proteintech, 10348- 1-AP; 1/100), and goat anti-Vimentin (Merck, Darmstadt, Germany, V4360; 1/150). .. After several washes, sections were incubated for 1 h at RT in a solution of PBS containing 0.3% Triton with the corresponding Alexa Fluor-conjugated secondary antibodies: Alexa Fluor 488 (Invitrogen, (Thermo Fisher Scientific) Waltham, MA, USA, A11029; 1/500), Alexa Fluor 594 (Invitrogen, A21207; 1/500), and Alexa Fluor 647 (Invitrogen, A32849; 1/500).

    Article Title: SLP2/PHB Aggregates in ALS Mouse Models and Patients: Implications Beyond CHCHD10 -Associated Motor Neuron Disease
    Article Snippet: .. Floating sections were incubated overnight at 4 °C with primary antibodies diluted in blocking buffer: mouse anti-PHB2 (Proteintech, Rosemont, IL, USA, 66424-1-Ig; 1/400), rabbit anti-SLP2 (Proteintech, 10348-1-AP; 1/100), and goat anti-Vimentin (Merck, Darmstadt, Germany, V4360; 1/150). .. After several washes, sections were incubated for 1 h at RT in a solution of PBS containing 0.3% Triton with the corresponding Alexa Fluor-conjugated secondary antibodies: Alexa Fluor 488 (Invitrogen, (Thermo Fisher Scientific) Waltham, MA, USA, A11029; 1/500), Alexa Fluor 594 (Invitrogen, A21207; 1/500), and Alexa Fluor 647 (Invitrogen, A32849; 1/500).

    Blocking Assay:

    Article Title: SLP2/PHB Aggregates in ALS Mouse Models and Patients: Implications Beyond CHCHD10-Associated Motor Neuron Disease
    Article Snippet: .. Floating sections were incubated overnight at 4 ◦C with primary antibodies diluted in blocking buffer: mouse anti-PHB2 (Proteintech, Rosemont, IL, USA, 66424-1-Ig; 1/400), rabbit anti-SLP2 (Proteintech, 10348- 1-AP; 1/100), and goat anti-Vimentin (Merck, Darmstadt, Germany, V4360; 1/150). .. After several washes, sections were incubated for 1 h at RT in a solution of PBS containing 0.3% Triton with the corresponding Alexa Fluor-conjugated secondary antibodies: Alexa Fluor 488 (Invitrogen, (Thermo Fisher Scientific) Waltham, MA, USA, A11029; 1/500), Alexa Fluor 594 (Invitrogen, A21207; 1/500), and Alexa Fluor 647 (Invitrogen, A32849; 1/500).

    Article Title: SLP2/PHB Aggregates in ALS Mouse Models and Patients: Implications Beyond CHCHD10 -Associated Motor Neuron Disease
    Article Snippet: .. Floating sections were incubated overnight at 4 °C with primary antibodies diluted in blocking buffer: mouse anti-PHB2 (Proteintech, Rosemont, IL, USA, 66424-1-Ig; 1/400), rabbit anti-SLP2 (Proteintech, 10348-1-AP; 1/100), and goat anti-Vimentin (Merck, Darmstadt, Germany, V4360; 1/150). .. After several washes, sections were incubated for 1 h at RT in a solution of PBS containing 0.3% Triton with the corresponding Alexa Fluor-conjugated secondary antibodies: Alexa Fluor 488 (Invitrogen, (Thermo Fisher Scientific) Waltham, MA, USA, A11029; 1/500), Alexa Fluor 594 (Invitrogen, A21207; 1/500), and Alexa Fluor 647 (Invitrogen, A32849; 1/500).

    Electrophoresis:

    Article Title: Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells
    Article Snippet: Samples were heated for 2–5 min at 95 ̊C and loaded on 10% Tris-glycine polyacryl- amide gels. .. After electrophoresis, proteins were electroblotted on low fluorescent PVDF or nitrocellulose membranes, and immunoblotted with the following primary antibodies: rabbit anti- OCIAD1 (Invitrogen, cat# PA5-20834, 1:2000–1:5000), mouse anti-OCIAD1 (Proteintech, cat# 66698–1-Ig, 1:5000), rabbit anti-OCIAD2 (Invitrogen, cat# PA5-59375, 1:500–1:5000), mouse anti- ATP5A1 (Proteintech, cat# 66037–1-Ig, 1:2000–1:5000), rabbit anti-NDUFB8 (Proteintech, cat# 14794–1-AP, 1:2000), mouse anti-SDHA (SantaCruz Biotechnology, cat# sc-166947, 1:2000–1:5000), rabbit anti-UQCRC2 (Proteintech, cat# 14742–1-AP, 1:2000–1:5000), mouse anti-UQCRC1 (Invitro- gen, cat# 459140, 1:2000), rabbit anti-CYC1 (Proteintech, cat# 10242–1-AP, 1:1000), mouse anti- COXIV (Proteintech, cat# 66110–1-1g, 1:2000), mouse anti-PHB2 (Proteintech, cat# 66424–1-Ig, 1:5000), rabbit anti-TIM50 (Proteintech, cat# 22229–1-AP, 1:1000), rabbit anti-TOM70 (Proteintech, cat# 14528–1-AP, 1:1000), mouse anti-GFP (Proteintech, cat# 66002–1-Ig, 1:2000), mouse anti-b- actin (Proteintech, cat# 66009–1-1g, 1:10,000), rabbit anti-GPD2 (Proteintech, cat# 17219–1-AP, 1:1000), rabbit anti-AIF (Proteintech, cat# 17984–1-AP, 1:4000). .. Secondary antibodies conjugated to DyLight 680 and DyLight 800 (Thermo Fisher Scientific, 1:5000) were used and visualized with an Odyssey Infrared Imaging System (LI-COR, Lincoln, NE).

    Article Title: Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells
    Article Snippet: .. After electrophoresis, proteins were electroblotted on low fluorescent PVDF or nitrocellulose membranes, and immunoblotted with the following primary antibodies: rabbit anti-OCIAD1 (Invitrogen, cat# PA5-20834, 1:2000–1:5000), mouse anti-OCIAD1 (Proteintech, cat# 66698–1-Ig, 1:5000), rabbit anti-OCIAD2 (Invitrogen, cat# PA5-59375, 1:500–1:5000), mouse anti-ATP5A1 (Proteintech, cat# 66037–1-Ig, 1:2000–1:5000), rabbit anti-NDUFB8 (Proteintech, cat# 14794–1-AP, 1:2000), mouse anti-SDHA (SantaCruz Biotechnology, cat# sc-166947, 1:2000–1:5000), rabbit anti-UQCRC2 (Proteintech, cat# 14742–1-AP, 1:2000–1:5000), mouse anti-UQCRC1 (Invitrogen, cat# 459140, 1:2000), rabbit anti-CYC1 (Proteintech, cat# 10242–1-AP, 1:1000), mouse anti-COXIV (Proteintech, cat# 66110–1-1g, 1:2000), mouse anti-PHB2 (Proteintech, cat# 66424–1-Ig, 1:5000), rabbit anti-TIM50 (Proteintech, cat# 22229–1-AP, 1:1000), rabbit anti-TOM70 (Proteintech, cat# 14528–1-AP, 1:1000), mouse anti-GFP (Proteintech, cat# 66002–1-Ig, 1:2000), mouse anti-β-actin (Proteintech, cat# 66009–1-1g, 1:10,000), rabbit anti-GPD2 (Proteintech, cat# 17219–1-AP, 1:1000), rabbit anti-AIF (Proteintech, cat# 17984–1-AP, 1:4000). .. Secondary antibodies conjugated to DyLight 680 and DyLight 800 (Thermo Fisher Scientific, 1:5000) were used and visualized with an Odyssey Infrared Imaging System (LI-COR, Lincoln, NE).

    Article Title: Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells
    Article Snippet: .. After electrophoresis, proteins were electroblotted on low fluorescent PVDF or nitrocellulose membranes, and immunoblotted with the following primary antibodies: rabbit anti-OCIAD1 (Invitrogen, cat# PA5-20834, 1:2000-1:5000), mouse anti-OCIAD1 (Proteintech, cat# 66698-1-Ig, 1:5000), rabbit anti-OCIAD2 (Invitrogen, cat# PA5-59375, 1:500-1:5000), mouse anti-ATP5A1 (Proteintech, cat# 66037-1-Ig, 1:2000-1:5000), rabbit anti-NDUFB8 (Proteintech, cat# 14794-1-AP, 1:2000), mouse anti-SDHA (SantaCruz Biotechnology, cat# sc-166947, 1:2000-1:5000), rabbit anti-UQCRC2 (Proteintech, cat# 14742-1-AP, 1:2000-1:5000), mouse anti-UQCRC1 (Invitrogen, cat# 459140, 1:2000), rabbit anti-CYC1 (Proteintech, cat# 10242-1-AP, 1:1000), mouse anti-COXIV (Proteintech, cat# 66110-1-1g, 1:2000), mouse anti-PHB2 (Proteintech, cat# 66424-1-Ig, 1:5000), rabbit anti-TIM50 (Proteintech, cat# 22229-1-AP, 1:1000), rabbit anti-TOM70 (Proteintech, cat# 14528-1-AP, 1:1000), mouse anti-GFP (Proteintech, cat# 66002-1-Ig, 1:2000), mouse anti-β-actin (Proteintech, cat# 66009-1-1g, 1:10000). .. Secondary antibodies conjugated to DyLight 680 and DyLight 800 (Thermo Fisher Scientific, 1:5000) were used and visualized with an Odyssey Infrared Imaging System (LI-COR, Lincoln, NE).



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    Characterization of NcGA7-binding proteins. (A) Silver staining for SDS–PAGE to identify NcGRA7-binding proteins by an anti-FLAG immunoprecipitation assay using 293T cells transfected with empty plasmid (Mock, Lanes 1 and 2) or NcGRA7 cDNA fused with a FLAG tag (Lanes 3 and 4). Enriched or exclusively detected proteins are shown as five bands, while the other three bands (#1, #2 and #3) were excluded from the MS analyses. M: molecular marker. Each experiment (biological replicate) was repeated two times. (B, C) Western blots of the fractions eluted from the anti-FLAG immunoprecipitate were analyzed by immunoblotting using antibodies against FLAG (B) , XPOT, XPO1, SLC25A13, DNAJA1, UQCRC2, PHB1, and <t>PHB2</t> (C) . 293T cells transfected with empty plasmid (E) or NcGRA7 cDNA fused with a FLAG tag (G7) at 20 h posttransfection and HFFs infected with the parental strain Nc1 of N. caninum (WT) and NcGRA7-complemented (7c) parasites at 40 h postinfection were used. Each experiment (similar biological replicate) was repeated two time (B, C) .
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    Characterization of NcGA7-binding proteins. (A) Silver staining for SDS–PAGE to identify NcGRA7-binding proteins by an anti-FLAG immunoprecipitation assay using 293T cells transfected with empty plasmid (Mock, Lanes 1 and 2) or NcGRA7 cDNA fused with a FLAG tag (Lanes 3 and 4). Enriched or exclusively detected proteins are shown as five bands, while the other three bands (#1, #2 and #3) were excluded from the MS analyses. M: molecular marker. Each experiment (biological replicate) was repeated two times. (B, C) Western blots of the fractions eluted from the anti-FLAG immunoprecipitate were analyzed by immunoblotting using antibodies against FLAG (B) , XPOT, XPO1, SLC25A13, DNAJA1, UQCRC2, PHB1, and PHB2 (C) . 293T cells transfected with empty plasmid (E) or NcGRA7 cDNA fused with a FLAG tag (G7) at 20 h posttransfection and HFFs infected with the parental strain Nc1 of N. caninum (WT) and NcGRA7-complemented (7c) parasites at 40 h postinfection were used. Each experiment (similar biological replicate) was repeated two time (B, C) .

    Journal: Frontiers in Immunology

    Article Title: Mitochondrial damage and IL-1β production in monocytes caused by Neospora caninum infection are mediated by dense granule protein 7 and prohibitins

    doi: 10.3389/fimmu.2025.1408992

    Figure Lengend Snippet: Characterization of NcGA7-binding proteins. (A) Silver staining for SDS–PAGE to identify NcGRA7-binding proteins by an anti-FLAG immunoprecipitation assay using 293T cells transfected with empty plasmid (Mock, Lanes 1 and 2) or NcGRA7 cDNA fused with a FLAG tag (Lanes 3 and 4). Enriched or exclusively detected proteins are shown as five bands, while the other three bands (#1, #2 and #3) were excluded from the MS analyses. M: molecular marker. Each experiment (biological replicate) was repeated two times. (B, C) Western blots of the fractions eluted from the anti-FLAG immunoprecipitate were analyzed by immunoblotting using antibodies against FLAG (B) , XPOT, XPO1, SLC25A13, DNAJA1, UQCRC2, PHB1, and PHB2 (C) . 293T cells transfected with empty plasmid (E) or NcGRA7 cDNA fused with a FLAG tag (G7) at 20 h posttransfection and HFFs infected with the parental strain Nc1 of N. caninum (WT) and NcGRA7-complemented (7c) parasites at 40 h postinfection were used. Each experiment (similar biological replicate) was repeated two time (B, C) .

    Article Snippet: Anti-FLAG M2 mouse monoclonal antibody (Sigma–Aldrich), anti-XPOT (Exportin-T) rabbit antibody (A303-972A, Bethyl, Montgomery, TX, USA), anti-XPO1(CRM1) rabbit antibody (A300-469A, Bethyl), anti-SLC25A13 rabbit antibody (GTX109001, GeneTex, Irvine, CA, USA), anti-DNAJA1 rabbit antibody (A304-516A, Bethyl), anti-UQCRC2 rabbit antibody (GTX114873, GeneTex), anti-PHB1 rabbit antibody (GTX101105, GeneTex), anti-CoxIV rabbit monoclonal antibody (3E11, Cell Signaling Technology, Danvers, MA, USA), anti-GAPDH rabbit monoclonal antibody (14C10, Cell Signaling Technology), anti-Mfn1 rabbit antibody (13798-1-AP, Proteintech, Rosemont, IL, USA), anti-AIF mouse monoclonal antibody (sc-13116, Santa Cruz Biotechnology, Dallas, TX, USA), anti-mtHSP70 mouse monoclonal antibody (JG1, Invitrogen, Waltham, MA, USA), anti-VDAC1 rabbit antibody (4866, Cell Signaling Technology), anti-PHB2 mouse monoclonal antibody (sc-133094, Santa Cruz Biotechnology), anti-Histone H3 rabbit antibody (9715S, Cell Signaling Technology), anti-phospho-NF-κB p65 rabbit monoclonal antibody (93H1, Cell Signaling Technology) and anti-NFκB p65 rabbit polyclonal antibody (sc-109, Santa Cruz Biotechnology) were used for western blot and indirect fluorescent antibody test (IFAT) in this study.

    Techniques: Binding Assay, Silver Staining, SDS Page, Immunoprecipitation, Transfection, Plasmid Preparation, FLAG-tag, Marker, Western Blot, Infection

    Localization of NcGRA7 in host mitochondria and binding to host mitochondrial proteins. (A) Schematic diagram of isolated mitochondria under isotonic and swelling conditions. Schematics of the isotonic (left) and swelling (right) conditions used in this study. Under isotonic conditions, proteinase K can access outer mitochondrial membrane (OMM) proteins but not the intermembrane space (IMS), inner mitochondrial membrane (IMM), or matrix proteins through membrane barriers. In addition, under swelling conditions, the protease can reach the IMS, followed by cleavage of both the OMM and IMS proteins. The inset shows mitochondrial proteins as markers for each category. (B) The mitochondrial fraction (Mt) isolated from HFFs was treated with proteinase K under isotonic (–) or hypotonic swelling (+) conditions. The reactants were developed by immunoblotting with antibodies against NcGRA7 or against several mitochondrial markers as indicated. OMM protein: Mfn1. IMS proteins: AIF, PHB1 and PHB2. IMM protein: COX IV. Matrix protein: mHsp70. Each experiment (biological replicate) was repeated two times. (C) Western blot of eluted fractions of HFFs infected with the parental strain Nc1 of N. caninum (WT) or NcGRA7-complemented parasites (7c) at 40 h post infection from anti-FLAG immunoprecipitation were analyzed by immunoblotting using antibodies against several mitochondrial markers as indicated. *Because mouse antibodies against FLAG, AIF and mHsp70 were used, the anti-mouse HRP secondary antibody was used to detect the anti-FLAG mouse antibody.

    Journal: Frontiers in Immunology

    Article Title: Mitochondrial damage and IL-1β production in monocytes caused by Neospora caninum infection are mediated by dense granule protein 7 and prohibitins

    doi: 10.3389/fimmu.2025.1408992

    Figure Lengend Snippet: Localization of NcGRA7 in host mitochondria and binding to host mitochondrial proteins. (A) Schematic diagram of isolated mitochondria under isotonic and swelling conditions. Schematics of the isotonic (left) and swelling (right) conditions used in this study. Under isotonic conditions, proteinase K can access outer mitochondrial membrane (OMM) proteins but not the intermembrane space (IMS), inner mitochondrial membrane (IMM), or matrix proteins through membrane barriers. In addition, under swelling conditions, the protease can reach the IMS, followed by cleavage of both the OMM and IMS proteins. The inset shows mitochondrial proteins as markers for each category. (B) The mitochondrial fraction (Mt) isolated from HFFs was treated with proteinase K under isotonic (–) or hypotonic swelling (+) conditions. The reactants were developed by immunoblotting with antibodies against NcGRA7 or against several mitochondrial markers as indicated. OMM protein: Mfn1. IMS proteins: AIF, PHB1 and PHB2. IMM protein: COX IV. Matrix protein: mHsp70. Each experiment (biological replicate) was repeated two times. (C) Western blot of eluted fractions of HFFs infected with the parental strain Nc1 of N. caninum (WT) or NcGRA7-complemented parasites (7c) at 40 h post infection from anti-FLAG immunoprecipitation were analyzed by immunoblotting using antibodies against several mitochondrial markers as indicated. *Because mouse antibodies against FLAG, AIF and mHsp70 were used, the anti-mouse HRP secondary antibody was used to detect the anti-FLAG mouse antibody.

    Article Snippet: Anti-FLAG M2 mouse monoclonal antibody (Sigma–Aldrich), anti-XPOT (Exportin-T) rabbit antibody (A303-972A, Bethyl, Montgomery, TX, USA), anti-XPO1(CRM1) rabbit antibody (A300-469A, Bethyl), anti-SLC25A13 rabbit antibody (GTX109001, GeneTex, Irvine, CA, USA), anti-DNAJA1 rabbit antibody (A304-516A, Bethyl), anti-UQCRC2 rabbit antibody (GTX114873, GeneTex), anti-PHB1 rabbit antibody (GTX101105, GeneTex), anti-CoxIV rabbit monoclonal antibody (3E11, Cell Signaling Technology, Danvers, MA, USA), anti-GAPDH rabbit monoclonal antibody (14C10, Cell Signaling Technology), anti-Mfn1 rabbit antibody (13798-1-AP, Proteintech, Rosemont, IL, USA), anti-AIF mouse monoclonal antibody (sc-13116, Santa Cruz Biotechnology, Dallas, TX, USA), anti-mtHSP70 mouse monoclonal antibody (JG1, Invitrogen, Waltham, MA, USA), anti-VDAC1 rabbit antibody (4866, Cell Signaling Technology), anti-PHB2 mouse monoclonal antibody (sc-133094, Santa Cruz Biotechnology), anti-Histone H3 rabbit antibody (9715S, Cell Signaling Technology), anti-phospho-NF-κB p65 rabbit monoclonal antibody (93H1, Cell Signaling Technology) and anti-NFκB p65 rabbit polyclonal antibody (sc-109, Santa Cruz Biotechnology) were used for western blot and indirect fluorescent antibody test (IFAT) in this study.

    Techniques: Binding Assay, Isolation, Membrane, Western Blot, Infection, Immunoprecipitation